Genomes 4 T.A. Brown 基因组（第四版） 英文原版PDF
《基因组4》已经过全面修订和更新。这是一本关于基因组和如何研究基因组的彻底的现代教科书。与《基因组3》一样，首先是技术，然后是基因组解剖学，接着是基因组功能，最后是基因组进化。涵盖了所有类型生物的基因组：病毒、细菌、真菌、植物以及包括人类和其他类人猿在内的动物。
基因组测序和组装方法已被彻底修订，包括对四个基因组项目的调查：人类、尼安德特人、大熊猫和大麦。基因组注释的覆盖范围强调全基因组RNA图谱，涵盖了CRISPR-Cas 9和确定基因功能的GWAS方法。从这些技术中获得的知识构成了三章的基础，这三章描述了三种主要类型的基因组：真核生物、原核生物（包括真核细胞器）和病毒（包括移动遗传元素）。对基因组表达和复制的报道是真正的基因组学，集中在DNA包装、表观基因组修饰、DNA结合蛋白、非编码RNA、调控基因组序列和蛋白质-蛋白质相互作用的全基因组影响。还包括转录组分析、代谢组学和系统生物学的应用。最后一章是基因组进化，重点介绍了表观基因组的进化，利用基因组学研究人类进化，以及利用群体基因组学推进植物育种。如果分子生物学的既定方法在今天仍有意义，就会被列入其中，而且总会解释为什么该方法仍很重要。
Genomes 4 has been completely revised and updated. It is a thoroughly modern textbook about genomes and how they are investigated. As with Genomes 3, techniques come first, then genome anatomies, followed by genome function, and finally genome evolution. The genomes of all types of organism are covered: viruses, bacteria, fungi, plants, and animals including humans and other hominids.
Genome sequencing and assembly methods have been thoroughly revised including a survey of four genome projects: human, Neanderthal, giant panda, and barley. Coverage of genome annotation emphasizes genome-wide RNA mapping, with CRISPR-Cas 9 and GWAS methods of determining gene function covered. The knowledge gained from these techniques forms the basis of the three chapters that describe the three main types of genomes: eukaryotic, prokaryotic (including eukaryotic organelles), and viral (including mobile genetic elements). Coverage of genome expression and replication is truly genomic, concentrating on the genome-wide implications of DNA packaging, epigenome modifications, DNA-binding proteins, non-coding RNAs, regulatory genome sequences, and protein-protein interactions. Also included are applications of transcriptome analysis, metabolomics, and systems biology. The final chapter is on genome evolution, focusing on the evolution of the epigenome, using genomics to study human evolution, and using population genomics to advance plant breeding. Established methods of molecular biology are included if they are still relevant today and there is always an explanation as to why the method is still important.
Each chapter has a set of short-answer questions, in-depth problems, and annotated further reading. There is also an extensive glossary. Genomes 4 is the ideal text for upper level courses focused on genomes and genomics.
